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KMID : 0379119970250030167
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Korean Journal of Mycology
1997 Volume.25 No. 3 p.167 ~ p.175
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Cloning of Two chitin Synthase Gene Fragments from Penicillium diversum
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이동훈/Lee, Dong Hun
이상근/배경숙/박희문/맹필재/조성필/Lee, Sang Keun/Bae, Kyung Sook/Park, Hee Moon/Maeng, Pil Jae/Cho, Seong Pil
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Abstract
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The PCR fragments of two distinct chitin synthase genes, PdCHS1 and PdCHS2, were doped from Penicillium diversum KCTC 6786. The nucleotide sequences of PdCHS1 and PdCHS2 contained uninterrupted open reading frames (ORFs) of 570 by excluding the primer sequence. The similarity analysis of the deduced amino acid sequences using BLASTP indicated that the possible evolutionary relationship between P. diversum and ascomycetous fungi. Multilignment of the deduced amino acid sequences of PdCHSs using CLASTAL W revealed that the PdCHSs fell into two different classes: PdCHS1 into Class I and PdCHS2 into Class II of chiton synthase defined by Bowen et al. (1992). By Southern blot analysis, it was shown that each of the two genes is present as a single copy in the genome of P. diversum KCTC 6786.
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